Special Section: Recent Advances in Silkworm Biology
نویسندگان
چکیده
Bombyx mori nucleopolyhedrosis virus (BmNPV), a natural pathogen of great economic significance in sericulture, has been exploited to generate recombinant baculoviruses harbouring genes of choice and achieve high level expression of cloned foreign genes. The BmNPV-based expression system offers the advantage of using the larval host rather than the insectderived cell lines for economic large-scale synthesis of biomolecules. Since commercial rearing of silkworms is routinely practised, a part of the silkworm stocks can be diverted to produce biomolecules other than silk and the more sophisticated tissue culture methodologies can be dispensed with on an industrial scale. Nonetheless, the BmNPV-based expression considerably lags behind the AcMNPV system in vogue today, in terms of the choice of expression-optimized cloning vectors and easy recombinant generation kits that are commercially available. This review provides a brief outline of the current state of knowledge on the basic biology and genomics of BmNPV and how the virus has been made use of to produce biomolecules through its larval host, the mulberry silkworm. Since hyper-transcription from the viral very late gene promoters forms the basis of high level expression through recombinant baculoviruses, some aspects of the viral gene transcription and the role of late gene expression factors have also been included in the review.
منابع مشابه
Special Section: Recent Advances in Silkworm Biology
The silkworm, Bombyx mori is infected by a number of viruses: Bombyx mori nuclear polyhedrosis virus (BmNPV), cytoplasmic polyhedrosis virus (CPV), infectious flacherie virus (IFV) and densonucleosis virus (DNV). Except DNV, resistance of silkworms to the virus is controlled by polygenes. Serologically, two DNV types, DNV-1 and DNV-2 are identified. The non-susceptibility to DNV types is confer...
متن کاملSpecial Section: Recent Advances in Silkworm Biology
The biology and genetics of silkworm, Bombyx mori, is the most advanced of any lepidopteran species. Its rich repertoire of genetic resources and potential applications in sericulture and as a model for other Lepidoptera led to the initiation of genomics research. During the past decade much effort has been made in the areas of marker development, and molecular maps have been constructed in sta...
متن کاملSpecial section: Selected papers from the Fourth International Workshop on Recent Advances in Monte Carlo Techniques for Radiation Therapy.
متن کامل
A review on the recent advances in the biology and aquaculture technology of Holothuria scabra
The highly valued sea cucumber Holothuria scabra is currently listed as endangered (EN) species in the IUCN Red List due to overfishing in most of its native locations, spurring the development of H. scabra aquaculture to ensure sustainability of the industry and species in the wild. This review presents a summary on the biology of Holothuria scabra and recent advancements of its aquaculture te...
متن کاملSpecial Section on Recent Advances in Integrated Photonic Devices
Welcome to the Special Section on Recent Advances in Integrated Photonic Devices. This special section has been planned to introduce the latest advances on integrated photonic devices and related technologies which offer a wide range of applications and are increasing in their importance. These technologies, including integrated photonic circuits, OEICs, micro-optics, and optical MEMS, are incr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2002